NAD(+)—diphthamide ADP-ribosyltransferase

NAD+-diphthamide ADP-ribosyltransferase
Identifiers
EC number	2.4.2.36
CAS number	52933-21-8
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles

In enzymology, a NAD+-diphthamide ADP-ribosyltransferase (EC 2.4.2.36) is an enzyme that catalyzes the chemical reaction

NAD⁺ + peptide diphthamide $\rightleftharpoons$ nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide

Thus, the two substrates of this enzyme are NAD⁺ and peptide diphthamide, whereas its two products are nicotinamide and peptide N-(ADP-D-ribosyl)diphthamide.

This enzyme belongs to the family of glycosyltransferases, to be specific, the pentosyltransferases. The systematic name of this enzyme class is NAD+:peptide-diphthamide N-(ADP-D-ribosyl)transferase. Other names in common use include ADP-ribosyltransferase, mono(ADPribosyl)transferase, and NAD-diphthamide ADP-ribosyltransferase.

Structural studies

As of late 2007, 15 structures have been solved for this class of enzymes, with PDB accession codes 1S5B, 1S5C, 1S5D, 1S5E, 1S5F, 1SGK, 1TOX, 1XDT, 1XK9, 1ZM3, 1ZM4, 1ZM9, 2A5D, 2A5F, and 2A5G.

References

• Lee H, Iglewski WJ (1984). "Cellular ADP-ribosyltransferase with the same mechanism of action as diphtheria toxin and Pseudomonas toxin A". Proc. Natl. Acad. Sci. U. S. A. 81 (9): 2703–7. doi:10.1073/pnas.81.9.2703. PMC 345138. PMID 6326138. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=345138. 
• Ueda K, Hayaishi O (1985). "ADP-ribosylation". Annu. Rev. Biochem. 54 (1): 73–100. doi:10.1146/annurev.bi.54.070185.000445. PMID 3927821. 

See also

• Diphtheria toxin

2.4.2: Pentosyl-
transferases

Ribose	ADP-ribosyltransferase NAD+:diphthamide ADP-ribosyltransferase (Diphtheria toxin) NAD(P)+:arginine ADP-ribosyltransferase (Pertussis toxin · Cholera toxin) Poly ADP ribose polymerase Sirtuin Phosphoribosyltransferase Adenine phosphoribosyltransferase · Hypoxanthine-guanine phosphoribosyltransferase · Uracil phosphoribosyltransferase · Amidophosphoribosyltransferase Other Purine nucleoside phosphorylase: Thymidine phosphorylase (ECGF1)
Other	Xylosyltransferase · Arabinosyltransferase (Indolylacetylinositol arabinosyltransferase)

2.4.99: Sialyl
transferases

Beta-galactoside alpha-2,6-sialyltransferase · Monosialoganglioside sialyltransferase · ST8SIA4

B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, 4.1/2/3/4/5/6, 5.1/2/3/4/99, 6.1-3/4/5-6