- Nicking enzyme
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A nicking enzyme (or nicking endonuclease) is an enzyme that cuts one strand of a double-stranded DNA at a specific recognition nucleotide sequences known as a restriction site. Such enzymes hydrolyse, cut, only one strand of the DNA duplex, to produce DNA molecules that are “nicked”, rather than cleaved. [1][2]
Their discovery can be used for strand-displacement amplification,[3] Nicking Enzyme Amplification Reaction, exonucleotyic degradation, or the creation of small gaps.[4] Over 200 nicking enzymes have been studied, and 13 of these are available commercially[5] and are routinely used for research and in commercial products.
References
- ^ Ando T, et. Al. (July 1969). "Isolation and characterization of enzymes with nicking action from phage T4-infected Escherichia coli". J Biochem. 66 (1): 1–10. PMID 4309718.
- ^ Morgan RD, Kong H., et al. (November 2000). "Characterization of the specific DNA nicking activity of restriction endonuclease N.BstNBI". Biol Chem. 381 (11): 1123–5. doi:10.1515/BC.2000.137. PMID 1154070.
- ^ Walker GT, Little MC, Nadeau JG, Shank DD. (Jan 1992). "Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system". Proc. Natl. Acad. Sci. U.S.A. 89 (1): 392–6. doi:10.1073/pnas.89.1.392. PMC 48243. PMID 1309614. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=48243.
- ^ Wang H, Hays JB. (October 2001). "Simple and rapid preparation of gapped plasmid DNA for incorporation of oligomers containing specific DNA lesions". Mol Biotechnol. 19 (2): 133–40. doi:10.1385/MB:19:2:133. PMID 11725483.
- ^ "REBASE Enzymes". Encyclopedia of restriction and nicking enzymes. http://rebase.neb.com/cgi-bin/azlist?nick. Retrieved 2009-06-01.
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