Quenching (fluorescence)


Quenching (fluorescence)

Quenching refers to any process which decreases the fluorescence intensity of a given substance. A variety of processes can result in quenching, such as excited state reactions, energy transfer, complex-formation and collisional quenching. As a consequence, quenching is often heavily dependent on pressure and temperature. Molecular oxygen and the iodide ion are common chemical quenchers. Quenching poses a problem for non-instant spectroscopic methods, such as laser-induced fluorescence.

Quenching is made use of in optode sensors; for instance the quenching effect of oxygen on certain rubidium complexes allows the measurement of oxygen saturation in solution. Quenching and dequenching upon interaction with a specific molecular biological target is the basis for activatable optical contrast agents for molecular imaging. [cite journal |author=Weissleder R, Tung CH, Mahmood U, Bogdanov A |title=In vivo imaging of tumors with protease-activated near-infrared fluorescent probes |journal=Nat. Biotechnol. |volume=17 |issue=4 |pages=375–8 |year=1999 |pmid=10207887 |doi=10.1038/7933]

ee also

* Quenching in Chemical Industries for extract product from Mother substance.
* Dark quencher, for use in molecular biology.
*Fluorescence resonance energy transfer, a phenomenon on which some quenching techniques rely

References


Wikimedia Foundation. 2010.

Look at other dictionaries:

  • Quenching (disambiguation) — Quenching may mean:* to quench, which is the rapid cooling of a solid to lock it into a metastable crystal structure * Quenching (fluorescence), any process which decreases the fluorescence intensity of a given substance * a loss of… …   Wikipedia

  • Fluorescence — Fluorescent minerals emit visible light when exposed to ultraviolet light Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation of a different wavelength.[1] It is a form of luminescence.… …   Wikipedia

  • fluorescence quenching — fluorescencijos gesinimas statusas T sritis fizika atitikmenys: angl. fluorescence quenching vok. Fluoreszenzauslöschung, f; Fluoreszenzlöschung, f rus. гашение флуоресценции, n pranc. extinction de fluorescence, f …   Fizikos terminų žodynas

  • quenching — 1. The process of extinguishing, removing, or diminishing a physical property such as heat or light; e.g., the cooling of a hot metal rapidly by plunging it into water or oil. 2. In beta liquid scintillation counting, the shifting of the energy… …   Medical dictionary

  • fluorescence quenching — a technique for measuring the primary interaction of antigen and antibody by determination of the amount of light absorbed by bound antigen from fluorescent labeled antibody exposed to ultraviolet radiation …   Medical dictionary

  • Chlorophyll fluorescence — is light that has been re emitted after being absorbed by chlorophyll molecules of plant leaves. By measuring the intensity and nature of this fluorescence, plant ecophysiology can be investigated. Contents 1 Assessing plant physiology with… …   Wikipedia

  • Non-photochemical quenching — (NPQ) is a mechanism employed by plants and algae to protect themselves from the adverse effects of high light intensity.[1] It involves the quenching of singlet excited state chlorophylls (Chl) via enhanced internal conversion to the ground… …   Wikipedia

  • homogeneous fluorescence immunoassay — a fluorescence immunoassay that is single phase and uses a change in fluorescence (such as quenching or increased polarization) accompanying antigen antibody binding to measure the amount of bound label without separation …   Medical dictionary

  • extinction de fluorescence — fluorescencijos gesinimas statusas T sritis fizika atitikmenys: angl. fluorescence quenching vok. Fluoreszenzauslöschung, f; Fluoreszenzlöschung, f rus. гашение флуоресценции, n pranc. extinction de fluorescence, f …   Fizikos terminų žodynas

  • Lipid bilayer fusion — Illustration of lipid vesicles fusing showing two possible outcomes: hemifusion and full fusion. In hemifusion only the outer bilayer leaflets mix. In full fusion both leaflets as well as the internal contents mix. Fusion is the process by which… …   Wikipedia


Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”

We are using cookies for the best presentation of our site. Continuing to use this site, you agree with this.