- Thermus aquaticus
Taxobox
name = "Thermus aquaticus"
image_width = 300px
Domain = Archaea
regnum =Bacteria
phylum =Deinococcus-Thermus
classis =Deinococci
ordo = Thermales
genus = "Thermus"
species = "T. aquaticus"
binomial = "Thermus aquaticus"
binomial_authority = Brock & Freeze, 1969"Thermus aquaticus" is a species of
bacterium that can tolerate high temperatures, one of several thermophilic bacteria that belong to theDeinococcus-Thermus group. It is the source of the heat-resistant enzyme Taq DNA Polymerase, one of the most important enzymes inmolecular biology because of its use in thepolymerase chain reaction (PCR) DNA amplification technique.History
When studies of biological organisms in hot springs began in the
1960 s, scientists thought that the life ofthermophilic bacteria could not be sustained in temperatures above about 55° Celsius (131° Fahrenheit) [Thomas Brock's essay "Life at High Temperatures", available at [http://www.bact.wisc.edu/themicrobialworld/LAHT/B1] ] . Soon, however, it was discovered that many bacteria in different springs not only survived but also thrived in higher temperatures. In1969 , Thomas Brock and Hudson Freeze of Indiana University reported a newspecies of thermophilicbacterium which they named "Thermus aquaticus" [Brock TD and Freeze H "Thermus aquaticus, a Nonsporulating Extreme Thermophile" J. Bact. vol. 98(1) pp.289-297 (Apr-1969).] . The bacterium was first discovered in the Great Fountain region ofYellowstone National Park , and has since been found in similar thermal habitats around the world.Biology
It thrives at 70°C (160°F), but can survive at temperatures of 50°C to 80°C (120°F to 175°F). This bacterium is a
chemotroph - it performschemosynthesis in order to obtain food. However, since its range of temperature overlaps somewhat with that of the photosyntheticcyanobacteria that share its ideal environment, it is sometimes found living in conjuncture with its neighbors, obtaining energy for growth from their photosynthesis.Enzymes from "T. aquaticus"
"T. aquaticus" has become famous as a source of thermostable enzymes, particularly the "Taq" DNA Polymerase, as described below.
:Aldolase:Studies of this extreme thermophilic bacterium that could be grown in
cell culture was initially centered on attempts to understand howprotein enzymes (which normally inactivate at high temperature) can function at high temperature inthermophile s. In 1970 Freeze and Brock published an article describing a thermostable aldolase enzyme from "Thermus aquaticus". [Freeze H and Brock TD "Thermostable Aldolase from Thermus aquaticus" J. Bact. vol. 101(2) pp. 541-550 (Feb-1970).]:RNA polymerase:The first
polymerase enzyme isolated from "Thermus aquaticus" in 1974, was a DNA-dependent RNA polymerase [Air GM and Harris JI "DNA-Dependent RNA Polymerase From the Thermophilic Bacterium Thermus aquaticus" FEBS Letters vol. 38(3) pp. 277-281 (1974).] , used in the process of Transcription.:Taq I restriction enzyme:Most molecular biologists probably became aware of "Thermus aquaticus" in the late 1970s or early 1980s because of the isolation of useful restriction endonucleases from this organism [cite journal|year=1978| first= S| last= Sato| title=A single cleavage of Simian virus 40 (SV40) DNA by a site specific endonuclease from Thermus aquaticus, Taq I| journal=J. Biochem (Tokyo)| month=Feb| volume=83| issue=2| pages=633–5] . Use of the term "Taq" to refer to "Thermus aquaticus" arose at this time from the convention of giving restriction enzymes short names such as Sal and Hin, names derived from the genus and species of the source organisms.
:DNA polymerase ("Taq pol"):DNA polymerase was first isolated from "Thermus aquaticus" in 1976 [cite journal| url=http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8432| title= Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus| first= A| last= Chien| coauthors= Edgar DB, and Trela JM| journal=J. Bact.| volume= 127| issue=3| pages= 1550–1557| pmid= 8432] . The first advantage that was found for this thermostable (temperature optimum 80°C) DNA polymerase was that it could be isolated in a purer form (free of other enzyme contaminants) than could the DNA polymerase from other sources. Later,
Kary Mullis and other investigators atCetus Corporation discovered that this enzyme could be used in thepolymerase chain reaction (PCR) process for amplifying short segments ofDNA [cite journal| last=Saiki| first= RK| coauthors= et al.| url=http://sunsite.berkeley.edu/cgi-bin/ebind2html/pcr/009| title= Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase| journal=Science| year= 1988| volume=239| pages= 487–91| doi= 10.1126/science.2448875| pmid= 2448875] , eliminating the need to add enzyme after every cycle of thermal denaturation of the DNA. The enzyme was also cloned, sequenced, modified (to produce the shorter 'Stoffel Fragment'), and produced in large quantities for commercial salecite journal|author=Lawyer FC et al.|year= 1993|title=High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase .. |journal= PCR Methods Appl.|volume=2|pages=275–287|pmid=8324500] . In 1989 "Science" magazine named Taq polymerase as its first "Molecule of the Year" [Science Magazine, Dec. 22, 1989, p. 1543.] . In 1993, Dr. Mullis was awarded the Nobel Prize for his work with PCR.:Other enzymes:The high optimum temperature for "Thermus aquaticus" allows researchers to study reactions under conditions for which other enzymes lose activity. Other enzymes isolated from this organism include
DNA ligase , Alkaline Phosphatase, NADH Oxidase, Isocitrate Dehydrogenase, Amylomaltase, and the ever-popular Fructose 1,6-Bisphosphate-Dependent L-Lactate Dehydrogenase.Controversy
The commercial use of enzymes from "T. aquaticus" has not been without controversy. After Dr. Brock's studies, samples of the organism were deposited in the
American Type Culture Collection , a public repository. Other scientists, including those at Cetus, obtained it from there. As the commercial potential of Taq Polymerase became apparent in the 1990s [Detailed history of Cetus and the commercial aspects of PCR. available at [http://www.j-biomed-discovery.com/content/1/1/7/ ] ] , theNational Park Service labeled its use as the "Great Taq Rip-off." [Robbins J " [http://www.nytimes.com/2006/11/28/science/28yell.html The Search for Private Profit in the Nation's Public Parks,]The New York Times ," Nov. 28, 2006.] Researchers working in National Parks are now required to sign "benefits sharing" agreements that would send a portion of later profits back to the Park Service.See also
*
Bacteria
*Biotechnology
*Thermophiles
*Geyser References
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