Thermolysin

Thermolysin

Thermolysin EC number|3.4.24.27 is a thermostable neutral metalloproteinase enzyme produced by the gram-positive bacteria "Bacillus thermoproteolyticus". [cite journal | author=Endo, S. | title= Studies on protease produced by thermophilic bacteria | journal= J. Ferment. Technol. | year=1962 | volume=40 | pages=346-353] It requires one zinc ion for enzyme activity and four calcium ions ions for structural stability. [cite journal | author=Tajima M, Urabe I. "et al". | title= Role of calcium ions in the thermostability of thermolysin and Bacillus subtilis var. amylosacchariticus neutral protease | journal= Eur. J. Biochem. | year=1976 | volume=64 | issue=1 | pages=243-247 | pmid=819262] Thermolysin specifically catalyzes the hydrolysis of peptide bonds containing hydrophobic amino acids. However thermolysin is also widely used for peptide bond formation through the reverse reaction of hydrolysis. [cite journal | author=Trusek-Holownia A. | title= Synthesis of ZAlaPheOMe, the precursor of bitter dipeptide in the two-phase ethyl acetate-water system catalysed by thermolysin | journal= J. Biotechnol. | year=2003 | volume=102 | issue=2 | pages=153-163 | pmid=12697393] Thermolysin is the most stable member of a family of metalloproteinases produced by various "Bacillus" species. These enzymes are also termed 'neutral' proteinases or thermolysin -like proteinases (TLPs).

ynthesis

Like all bacterial extracellular proteases thermolysin is first synthesised by the bactrium as a pre-proenzyme. [cite journal | author=Yasukawa K, Kusano M, Inouye K. | title= A new method for the extracellular production of recombinant thermolysin by co-expressing the mature sequence and pro-sequence in Escherichia coli | journal= Protein Eng. Des. Sel. | year=2007 | volume=20 | issue=8 | pages=375-383| pmid=17616558] Thermolysin is synthesized as a pre-proenzyme consisting of a signal peptide 28 amino acids long, a pro-peptide 204 amino acids long and the mature enzyme itself 316 amino acids in length. The signal peptide acts as a signal for translocation of pre-prothermolysin to the bacterial cytoplasmic membrane. In the periplasm pre-prothermolysin is then processed into prothermolysin by a signal peptidase. The prosequence then acts as a molecular chaperone and leads to autocleavage of the peptide bond linking pro and mature sequences. The mature protein is then secreted into the extracellular medium. [cite journal | author=Inouye K, Kusano M. "et al". | title=Engineering, expression, purification, and production of recombinant thermolysin | journal=Biotechnol. Annu. Rev. | year=2007 | volume=13 | pages=43-64 | pmid=17875473]

tructure


[


left|thumb|300px|right|Structure_of_thermolysin_taken_from_pdb file [http://www.pdb.org/pdb/explore.do?structureId=3TMN 3TMN] ]
Thermolysin consists of a beta pleated sheet rich N-terminal domain and a alpha helical rich C-terminal domain. These two domains are connected by a central alpha helix.

References

External links

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Look at other dictionaries:

  • Thermolysin — Bändermodell nach PDB  …   Deutsch Wikipedia

  • Thermolysin — Ther mo*ly sin, n. [Thermo + Gr. ly ein to loose + in.] (Biochem.) a proteolytic enzyme obtained from the bacterium {Bacillus thermoproteolyticus}, which hydrolyses the N terminal amide bonds of hydrophobic amino acid residues in proteins. It is… …   The Collaborative International Dictionary of English

  • thermolysin — see thermo …   Useful english dictionary

  • thermolysin — termolizinas statusas T sritis chemija apibrėžtis Šilumai atsparus Zn²⁺ ir Ca²⁺ turintis proteolizinis fermentas. atitikmenys: angl. thermolysin rus. термолизин …   Chemijos terminų aiškinamasis žodynas

  • thermolysin — Heat stable metalloproteinase (EC 3.4.24.4.) produced by a strain of Bacillus stearothermophilus. Retains 50% of its activity after 1h at 80°C …   Dictionary of molecular biology

  • thermolysin — noun A thermostable neutral metalloproteinase enzyme produced by the gram positive bacterium Bacillus thermoproteolyticus …   Wiktionary

  • Thermolysin — Ther|mo|ly|sin [↑ Lysin (2)], das; s, e: eine bakterielle, bis 80 °C biol. aktive Zink Proteinase mit 316 Aminosäureresten u. MG 37 500, die haupts. Phe u. Leu Reste hydrolysiert. T. dient u. a. zur Aspartam Synthese …   Universal-Lexikon

  • thermolysin — ther·mol·y·sin thər mäl ə sən n a zinc containing microbial enzyme that is used to catalyze the hydrolysis of proteins esp. when it is desired to keep the disulfide bonds intact …   Medical dictionary

  • Bacillus thermoproteolyticus — Thermolysin Ther mo*ly sin, n. [Thermo + Gr. ly ein to loose + in.] (Biochem.) a proteolytic enzyme obtained from the bacterium {Bacillus thermoproteolyticus}, which hydrolyses the N terminal amide bonds of hydrophobic amino acid residues in… …   The Collaborative International Dictionary of English

  • Peptidasen — Peptidase Enzymklassifikation EC, Kategorie …   Deutsch Wikipedia

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