NEDD4L

Neural precursor cell expressed, developmentally down-regulated 4-like
PDB rendering based on 1wr3.
Available structures PDB 2NSQ, 2ONI, 3JVZ, 3JW0
Identifiers
Symbols	NEDD4L; FLJ33870; KIAA0439; NEDD4-2; RSP5; hNedd4-2
External IDs	OMIM: 606384 MGI: 1933754 HomoloGene: 86986 GeneCards: NEDD4L Gene
EC number	6.3.2.-
Gene Ontology Molecular function • ubiquitin-protein ligase activity • ubiquitin-protein ligase activity • protein binding • ligase activity • acid-amino acid ligase activity • sodium channel regulator activity Cellular component • intracellular • cytoplasm Biological process • protein modification process • sodium ion transport • cellular sodium ion homeostasis • excretion • response to metal ion • negative regulation of sodium ion transport • protein ubiquitination • water homeostasis • regulation of protein catabolic process • protein ubiquitination involved in ubiquitin-dependent protein catabolic process • interspecies interaction between organisms • positive regulation of endocytosis Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	23327	83814
Ensembl	ENSG00000049759	ENSMUSG00000024589
UniProt	Q96PU5	Q8CFI0
RefSeq (mRNA)	NM_001144964.1	NM_031881
RefSeq (protein)	NP_001138436.1	NP_114087
Location (UCSC)	Chr 18: 55.71 – 56.07 Mb	Chr 18: 65.05 – 65.38 Mb
PubMed search	[1]	[2]

E3 ubiquitin-protein ligase NEDD4-like is an enzyme that in humans is encoded by the NEDD4L gene.^[1][2][3][4]

Interactions

NEDD4L has been shown to interact with SCNN1A.^[2][5]

References

1. ^ Erdeniz N, Rothstein R (Jan 2000). "Rsp5, a Ubiquitin-Protein Ligase, Is Involved in Degradation of the Single-Stranded-DNA Binding Protein Rfa1 in Saccharomyces cerevisiae". Mol Cell Biol 20 (1): 224–32. doi:10.1128/MCB.20.1.224-232.2000. PMC 85078. PMID 10594025. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=85078. 
2. ^ ^{a b} Harvey KF, Dinudom A, Cook DI, Kumar S (May 2001). "The Nedd4-like protein KIAA0439 is a potential regulator of the epithelial sodium channel". J Biol Chem 276 (11): 8597–601. doi:10.1074/jbc.C000906200. PMID 11244092. 
3. ^ Raikwar NS, Thomas CP (May 2008). "Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel". Am J Physiol Renal Physiol 294 (5): F1157–65. doi:10.1152/ajprenal.00339.2007. PMC 2424110. PMID 18322022. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2424110. 
4. ^ "Entrez Gene: NEDD4L Neural precursor cell expressed, developmentally down-regulated 4-like". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=23327. 
5. ^ Malbert-Colas, Laurence; Nicolas Gaël, Galand Colette, Lecomte Marie-Christine, Dhermy Didier (Jul. 2003). "Identification of new partners of the epithelial sodium channel alpha subunit". C. R. Biol. (France) 326 (7): 615–24. ISSN 1631-0691. PMID 14556380. 

Further reading

• Cook DI, Dinudom A, Komwatana P et al. (2003). "Patch-clamp studies on epithelial sodium channels in salivary duct cells". Cell Biochem. Biophys. 36 (2–3): 105–13. doi:10.1385/CBB:36:2-3:105. PMID 12139396. 
• Nuber U, Schwarz S, Kaiser P et al. (1996). "Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5". J. Biol. Chem. 271 (5): 2795–800. doi:10.1074/jbc.271.5.2795. PMID 8576257. 
• Ishikawa K, Nagase T, Nakajima D et al. (1998). "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 4 (5): 307–13. doi:10.1093/dnares/4.5.307. PMID 9455477. 
• Winberg G, Matskova L, Chen F et al. (2000). "Latent Membrane Protein 2A of Epstein-Barr Virus Binds WW Domain E3 Protein-Ubiquitin Ligases That Ubiquitinate B-Cell Tyrosine Kinases". Mol. Cell. Biol. 20 (22): 8526–35. doi:10.1128/MCB.20.22.8526-8535.2000. PMC 102158. PMID 11046148. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=102158. 
• Kikonyogo A, Bouamr F, Vana ML et al. (2001). "Proteins related to the Nedd4 family of ubiquitin protein ligases interact with the L domain of Rous sarcoma virus and are required for gag budding from cells". Proc. Natl. Acad. Sci. U.S.A. 98 (20): 11199–204. doi:10.1073/pnas.201268998. PMC 58707. PMID 11562473. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=58707. 
• Snyder PM, Olson DR, Thomas BC (2002). "Serum and glucocorticoid-regulated kinase modulates Nedd4-2-mediated inhibition of the epithelial Na+ channel". J. Biol. Chem. 277 (1): 5–8. doi:10.1074/jbc.C100623200. PMID 11696533. 
• Debonneville C, Flores SY, Kamynina E et al. (2002). "Phosphorylation of Nedd4-2 by Sgk1 regulates epithelial Na+ channel cell surface expression". EMBO J. 20 (24): 7052–9. doi:10.1093/emboj/20.24.7052. PMC 125341. PMID 11742982. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=125341. 
• Harvey KF, Shearwin-Whyatt LM, Fotia A et al. (2002). "N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein". J. Biol. Chem. 277 (11): 9307–17. doi:10.1074/jbc.M110443200. PMID 11748237. 
• Chen H, Ross CA, Wang N et al. (2002). "NEDD4L on human chromosome 18q21 has multiple forms of transcripts and is a homologue of the mouse Nedd4-2 gene". Eur. J. Hum. Genet. 9 (12): 922–30. doi:10.1038/sj.ejhg.5200747. PMID 11840194. 
• Konstas AA, Shearwin-Whyatt LM, Fotia AB et al. (2002). "Regulation of the epithelial sodium channel by N4WBP5A, a novel Nedd4/Nedd4-2-interacting protein". J. Biol. Chem. 277 (33): 29406–16. doi:10.1074/jbc.M203018200. PMID 12050153. 
• Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Dunn DM, Ishigami T, Pankow J et al. (2003). "Common variant of human NEDD4L activates a cryptic splice site to form a frameshifted transcript". J. Hum. Genet. 47 (12): 665–76. doi:10.1007/s100380200102. PMID 12522688. 
• Asher C, Sinha I, Garty H (2003). "Characterization of the interactions between Nedd4-2, ENaC, and sgk-1 using surface plasmon resonance". Biochim. Biophys. Acta 1612 (1): 59–64. doi:10.1016/S0005-2736(03)00083-X. PMID 12729930. 
• Itani OA, Campbell JR, Herrero J et al. (2003). "Alternate promoters and variable splicing lead to hNedd4-2 isoforms with a C2 domain and varying number of WW domains". Am. J. Physiol. Renal Physiol. 285 (5): F916–29. doi:10.1152/ajprenal.00203.2003. PMID 12876068. 
• Boehmer C, Henke G, Schniepp R et al. (2003). "Regulation of the glutamate transporter EAAT1 by the ubiquitin ligase Nedd4-2 and the serum and glucocorticoid-inducible kinase isoforms SGK1/3 and protein kinase B". J. Neurochem. 86 (5): 1181–8. doi:10.1046/j.1471-4159.2003.01937.x. PMID 12911626. 
• Malbert-Colas L, Nicolas G, Galand C et al. (2004). "Identification of new partners of the epithelial sodium channel alpha subunit". C. R. Biol. 326 (7): 615–24. PMID 14556380. 
• Qi H, Grenier J, Fournier A, Labrie C (2004). "Androgens differentially regulate the expression of NEDD4L transcripts in LNCaP human prostate cancer cells". Mol. Cell. Endocrinol. 210 (1–2): 51–62. doi:10.1016/j.mce.2003.08.009. PMID 14615060.