- DNA-(apurinic or apyrimidinic site) lyase
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DNA-(apurinic or apyrimidinic site) lyase Identifiers EC number 4.2.99.18 CAS number 61811-29-8 Databases IntEnz IntEnz view BRENDA BRENDA entry ExPASy NiceZyme view KEGG KEGG entry MetaCyc metabolic pathway PRIAM profile PDB structures RCSB PDB PDBe PDBsum Gene Ontology AmiGO / EGO Search PMC articles PubMed articles In enzymology, a DNA-(apurinic or apyrimidinic site) lyase (EC 4.2.99.18) is an enzyme that catalyzes the chemical reaction
- The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
This enzyme belongs to the family of lyases, specifically the "catch-all" class of lyases that cleave carbon-oxygen bonds. The systematic name of this enzyme class is DNA-(apurinic or apyrimidinic site) 5'-phosphomonoester-lyase. Other names in common use include AP lyase, AP endonuclease class I, endodeoxyribonuclease (apurinic or apyrimidinic), deoxyribonuclease (apurinic or apyrimidinic), E. coli endonuclease III, phage-T4 UV endonuclease, Micrococcus luteus UV endonuclease, AP site-DNA 5'-phosphomonoester-lyase, and X-ray endonuclease III.
Structural studies
As of late 2007, 23 structures have been solved for this class of enzymes, with PDB accession codes 1BIX, 1CQG, 1CQH, 1DE8, 1DE9, 1DEW, 1E9N, 1HD7, 1N39, 1N3A, 1N3C, 1VYB, 2ABK, 2I5W, 2ISI, 2J63, 2NOB, 2NOE, 2NOF, 2NOH, 2NOI, 2NOL, and 2NOZ.
References
- Bailly V, Sente B, Verly WG (1989). "Bacteriophage-T4 and Micrococcus luteus UV endonucleases are not endonucleases but beta-elimination and sometimes beta delta-elimination catalysts". Biochem. J. 259 (3): 751–9. PMC 1138582. PMID 2471512. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1138582.
- Bailly V, Verly WG (1987). "Escherichia coli endonuclease III is not an endonuclease but a beta-elimination catalyst". Biochem. J. 242 (2): 565–72. PMC 1147742. PMID 2439070. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1147742.
- Bailly V, Verly WG (1989). "AP endonucleases and AP lyases". Nucleic. Acids. Res. 17 (9): 3617–8. doi:10.1093/nar/17.9.3617. PMC 317828. PMID 2471157. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=317828.
- PH; Mazumder, Abhijit.; Ransom, Stephen C.; Gerlt, John A.; Bolton, Philip H. (1988). "Mechanism of UV endonuclease-V cleavage of abasic sites in DNA determined by C-13 labeling". J. Am. Chem. Soc. 110 (8): 2690–2691. doi:10.1021/ja00216a074.
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