NLRC4

NLR family, CARD domain containing 4
Identifiers
Symbols	NLRC4; CARD12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; IPAF
External IDs	OMIM: 606831 MGI: 3036243 HomoloGene: 10924 GeneCards: NLRC4 Gene
Gene Ontology Molecular function • nucleotide binding • magnesium ion binding • protein binding • ATP binding • protein homodimerization activity Cellular component • intracellular • cytoplasm Biological process • activation of caspase activity • detection of bacterium • defense response to bacterium • positive regulation of apoptosis • interleukin-1 beta secretion Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	58484	268973
Ensembl	ENSG00000091106	ENSMUSG00000039193
UniProt	Q9NPP4	n/a
RefSeq (mRNA)	NM_001199138.1	NM_001033367.3
RefSeq (protein)	NP_001186067.1	NP_001028539.1
Location (UCSC)	Chr 2: 32.45 – 32.49 Mb	Chr 17: 74.83 – 74.86 Mb
PubMed search	[1]	[2]

NLR family CARD domain-containing protein 4 is a protein that in humans is encoded by the NLRC4 gene.^[1][2]

In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1 (MIM 602233), NOD1 (CARD4; MIM 605980), and NOD2 (CARD15; MIM 605956). These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.[supplied by OMIM]^[2]

Interactions

NLRC4 has been shown to interact with caspase 1^[3][4] and NOD2.^[3][4]

References

1. ^ Geddes BJ, Wang L, Huang WJ, Lavellee M, Manji GA, Brown M, Jurman M, Cao J, Morgenstern J, Merriam S, Glucksmann MA, DiStefano PS, Bertin J (May 2001). "Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis". Biochem Biophys Res Commun 284 (1): 77–82. doi:10.1006/bbrc.2001.4928. PMID 11374873. 
2. ^ ^{a b} "Entrez Gene: NLRC4 NLR family, CARD domain containing 4". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=58484. 
3. ^ ^{a b} Damiano, Jason S; Oliveira Vasco, Welsh Kate, Reed John C (Jul. 2004). "Heterotypic interactions among NACHT domains: implications for regulation of innate immune responses". Biochem. J. (England) 381 (Pt 1): 213–9. doi:10.1042/BJ20031506. PMC 1133779. PMID 15107016. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1133779. 
4. ^ ^{a b} Damiano, J S; Stehlik C, Pio F, Godzik A, Reed J C (Jul. 2001). "CLAN, a novel human CED-4-like gene". Genomics (United States) 75 (1-3): 77–83. doi:10.1006/geno.2001.6579. ISSN 0888-7543. PMID 11472070. 

Further reading

• Poyet JL, Srinivasula SM, Tnani M, et al. (2001). "Identification of Ipaf, a human caspase-1-activating protein related to Apaf-1.". J. Biol. Chem. 276 (30): 28309–13. doi:10.1074/jbc.C100250200. PMID 11390368. 
• Damiano JS, Stehlik C, Pio F, et al. (2001). "CLAN, a novel human CED-4-like gene.". Genomics 75 (1-3): 77–83. doi:10.1006/geno.2001.6579. PMID 11472070. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Fu WN, Bertoni F, Kelsey SM, et al. (2003). "Role of DNA methylation in the suppression of Apaf-1 protein in human leukaemia.". Oncogene 22 (3): 451–5. doi:10.1038/sj.onc.1206147. PMID 12545166. 
• Clark HF, Gurney AL, Abaya E, et al. (2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=403697. 
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039. 
• Agostini L, Martinon F, Burns K, et al. (2004). "NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder.". Immunity 20 (3): 319–25. doi:10.1016/S1074-7613(04)00046-9. PMID 15030775. 
• Damiano JS, Oliveira V, Welsh K, Reed JC (2004). "Heterotypic interactions among NACHT domains: implications for regulation of innate immune responses.". Biochem. J. 381 (Pt 1): 213–9. doi:10.1042/BJ20031506. PMC 1133779. PMID 15107016. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1133779. 
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928. 
• Damiano JS, Newman RM, Reed JC (2004). "Multiple roles of CLAN (caspase-associated recruitment domain, leucine-rich repeat, and NAIP CIIA HET-E, and TP1-containing protein) in the mammalian innate immune response.". J. Immunol. 173 (10): 6338–45. PMID 15528373. 
• Sadasivam S, Gupta S, Radha V, et al. (2005). "Caspase-1 activator Ipaf is a p53-inducible gene involved in apoptosis.". Oncogene 24 (4): 627–36. doi:10.1038/sj.onc.1208201. PMID 15580302. 
• Lu C, Wang A, Wang L, et al. (2005). "Nucleotide binding to CARD12 and its role in CARD12-mediated caspase-1 activation.". Biochem. Biophys. Res. Commun. 331 (4): 1114–9. doi:10.1016/j.bbrc.2005.04.027. PMID 15882992. 
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. 
• Thalappilly S, Sadasivam S, Radha V, Swarup G (2006). "Involvement of caspase 1 and its activator Ipaf upstream of mitochondrial events in apoptosis.". FEBS J. 273 (12): 2766–78. doi:10.1111/j.1742-4658.2006.05293.x. PMID 16817903.