Henderson limit

Henderson limit

The value of the Henderson limit is defined as 2 × 107 Gy (J/kg).

Although generalizable, the limit is defined in the context of biomolecular X-ray crystallography, where a typical experiment consists of exposing a single frozen crystal of a macromolecule (generally protein, DNA or RNA) to an intense X-ray beam. The beams that are diffracted are then analyzed towards obtaining an atomically resolved model of the crystal.

The limit is defined as the X-ray dose (energy per unit mass) a cryo-cooled crystal can absorb before the diffraction pattern decays to half of its original intensity. Such decay presents itself as a problem for crystallographers who require that the diffraction intensities decay as little as possible, to maximize the signal to noise ratio in order to determine accurate atomic models that describe the crystal.

Although the process is still not fully understood, diffraction patterns of crystals typically decay with X-ray exposure due to a number of processes which non uniformly and irreversibly modify molecules that compose the crystal. These modifications induce disorder and thus decrease the intensity of Bragg diffraction. The processes behind these modifications include primary damage via the photo electric effect, covalent modification by free radicals, oxidation (methionine residues), reduction (disulfide bonds) and decarboxylation (glutamate, aspartate residues).


* Henderson, R. (1990). Proc. R. Soc. London, B241, 6-8.

Wikimedia Foundation. 2010.

Look at other dictionaries:

  • Henderson County, North Carolina — US County infobox county = Henderson County state = North Carolina map size = 250 founded = 1838 seat = Hendersonville | area total sq mi =375 area water sq mi =1 area percentage = 0.28% census yr = 2000 pop = 89173 density km2 =92 web =… …   Wikipedia

  • Zach Henderson — infobox poker player firstname=Zach lastname=Henderson nickname=Zach6668, Boats Henderson hometown=Ottawa, Ontario wsop bracelet count=0 wsop money finishes=1 wpt titles= None wpt final tables= None wpt money finishes=0 ept titles= None ept final …   Wikipedia

  • Dan Henderson — Born Daniel Jeffery Henderson August 24, 1970 …   Wikipedia

  • Hart am Limit — Filmdaten Deutscher Titel Hart am Limit Originaltitel Torque Produktionsl …   Deutsch Wikipedia

  • Douglas Henderson (ambassador) — Douglas Henderson (October 15, 1914 July 14, 2010) was an American diplomat, economist and government official. He is perhaps best known for his service as United States Ambassador to Bolivia during Marxist revolutionary Ernesto “Che” Guevara s… …   Wikipedia

  • Frank Henderson — Infobox poker player imagesize = 150px firstname=Frank lastname=Henderson nickname=Hollywood |caption= hometown=Houston, Texas wsop bracelet count=1 wsop money finishes=35 wsop main event best finish rank=2nd wsop main event best finish year=1987 …   Wikipedia

  • Sir James Henderson-Stewart, 1st Baronet — Sir James Henderson Stewart, 1st Baronet, (originally James Henderson Stewart, 6 December 1897 ndash; 3 September 1961) was a British banker, Army officer and politician. He was a National Liberal Member of Parliament for East Fife from 1933… …   Wikipedia

  • Toby Henderson — Cyclist infobox ridername = Toby Henderson fullname = Tobias S. Henderson nickname = Coca Cola Cowboy The California Kid dateofbirth = birth date and age |1961|10|10 height = 1.83m (6 0 Imperial) weight = 78kg (172lbs. Imperial) country = USA… …   Wikipedia

  • Martin Henderson — (2005) Martin Henderson (* 8. Oktober 1974 in Auckland, Neuseeland) ist ein neuseeländischer Schauspieler. Leben und Karriere Er begann seine Schauspielkarriere mit Rollen in neuseeländischen Film und Fernsehproduktionen. Von 1992 bis 1995… …   Deutsch Wikipedia

  • Melvin Starkey Henderson — (1883–1954) was an American orthopedic surgeon, who was born in St. Paul, Minnesota. Biography Upon his father s death, he went to Winnipeg, Manitoba to live with relatives, the family of Edward Drewry and owner of the Drewry Brewery. There he… …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”

We are using cookies for the best presentation of our site. Continuing to use this site, you agree with this.