Expression vector

Expression vector

An expression vector, otherwise known as an expression construct, is generally a plasmid that is used to introduce and express a specific gene into a target cell. Expression vector allows production of large amounts of stable mRNA. Once the expression vector is inside the cell, the protein that is encoded by the gene is produced by the cellular transcription and translation machinery. The plasmid is engineered such that it contains a highly active promoter which causes the production of large amounts of mRNA.

After expression of the gene product, the purification of the protein is required; but since the vector is introduced to a host cell, the protein of interest should be purified from the proteins of the host cell. Therefore, to make purification process easy, the cloned gene should have a tag. This tag could be histidine (His) tag or any other marker peptide.

Expression vectors are used for molecular biology techniques such as site-directed mutagenesis. In general, DNA vectors that are used in many molecular biology gene cloning experiments need not result in the expression of a protein. Expression vectors are often specifically designed to contain regulatory sequences that act as enhancer and promoter regions, and lead to efficient transcription of the gene that is carried on the expression vector. Expression vectors are basic tools for biotechnology and the production of proteins such as insulin that are important for medical treatments of specific diseases like diabetes.

References

*Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P (2002) "Molecular Biology of the Cell", 4th Edition. Garland Science ISBN 0-8153-4072-9.


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Look at other dictionaries:

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  • expression vector — expression vector. См. экспрессионный вектор. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

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  • expression vector — A vector that results in the expression of inserted DNA sequences when propagated in a suitable host cell, ie. the protein coded for by the DNA is synthesized by the host s system …   Dictionary of molecular biology

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  • expression vector — a cloning vector designed such that the inserted DNA is efficiently transcribed and translated in the host cell …   Medical dictionary

  • expression vector — A cloning vector that has been constructed in such a way that, after insertion of a DNA molecule, its coding sequence is properly transcribed and the mRNA is translated. The cloned gene is put under the control of a promoter sequence for the… …   Glossary of Biotechnology

  • Vector — may refer to: In mathematics * Euclidean vector, a geometric entity endowed with both length and direction, an element of a Euclidean vector space * Coordinate vector, in linear algebra, an explicit representation of an element of any abstract… …   Wikipedia

  • Expression cloning — is a technique in DNA cloning that uses expression vectors to generate a library of clones, with each clone expressing one protein. This expression library is then screened for the property of interest and clones of interest recovered for further …   Wikipedia

  • expression cloning vector — expression vector (см.). (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

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