Laser microdissection

Laser microdissection

Laser microdissection is an extraction process to dissect specific tissue for analysis or research.

Extraction process

A laser is coupled into a microscope and focuses onto the tissue of the slide. By movement of the laser by optics or the stage the focus follows a trajectory which is predefined by the user. This trajectory, a so called "Element", is then cut out and separated from the adjacent tissue. After the cutting process, an extraction process has to follow if an extraction process is desired.

Theoretically, there are several ways to extract tissue from a microscope slide with a histopathology sample on it:

* Press a sticky surface onto the sample and tear out. This will extract the desired region, but also bears the chance to carry particles or unwanted tissue on the surface, because an "allround sticky" surface is not selective.
* Melt a plastic membrane onto the sample and tear out. The heat is introduced by an, e.g., red or IR laser onto a membrane stained with an absorbing dye. As this adheres the desired sample onto the membrane, as with any membrane that is put close to the histopathology sample surface, there might be some debris extracted. Another danger is the introduced heat: Some molecules like DNA, RNA, or protein don't allow to be heated too much or at all for the goal of being isolated as purely as possible.
* Transport without contact. There are two different approaches:
#Transport simply by gravity using an upright microscope or
#Transport by "Laser Pressure Catapult" destroying parts of the tissue for the transport.

External links


* [http://www.leica-microsystems.com/lmd6000 Leica Microsystems] commercial non-contact Laser Microdissection and Imaging Workstations
* [http://home.ccr.cancer.gov/LOP/Research/lcm/Default.asp National Institutes of Health Laser Microdissection Core Facility]


Wikimedia Foundation. 2010.

Игры ⚽ Нужен реферат?

Look at other dictionaries:

  • Laser capture microdissection — (LCM) is a method for isolating pure cells of interest from specific microscopic regions of tissue sections.cite journal |author=Emmert Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang Z, Goldstein SR, Weiss RA, Liotta LA |title=Laser capture… …   Wikipedia

  • Microdissection — Intervention MeSH D042282 Microdissection refers to a variety of techniques where a microscope is used to assist in dissection. Different kinds of techniques involve microdissection …   Wikipedia

  • Laser-Mikrodissektion — Die Laser Mikrodissektion ist eine mikroskopische Technik zur lasergestützten Mikrodissektion von Geweben und Zellen. Inhaltsverzeichnis 1 Allgemeines 2 Anwendungsgebiete 3 Systeme …   Deutsch Wikipedia

  • Laser Mikrodissektion — Die Laser Mikrodissektion ist eine mikroskopische Technik zur lasergestützten Mikrodissektion von Geweben und Zellen. Inhaltsverzeichnis 1 Allgemeines 2 Anwendungsgebiete 3 Systeme 4 Einzelnachweise …   Deutsch Wikipedia

  • Laser — For other uses, see Laser (disambiguation). United States Air Force laser experiment …   Wikipedia

  • Laser applications — There are many scientific, military, medical and commercial laser applications which have been developed since the invention of the laser in the 1958. The coherency, high monochromaticity, and ability to reach extremely high powers are all… …   Wikipedia

  • Chromosome microdissection — Left panels show metaphase chromosomes before microdissection (top) and after a chromosomal band is removed (bottom). Right panels show an illustration of the same information for clarity. Chromosome microdissection is a technique that physically …   Wikipedia

  • Microtome — A microtome used in microscopy. A microtome (from the Greek mikros, meaning small , and temnein, meaning to cut ) is a sectioning instrument that allows for the cutting of extremely thin slices of material, known as sections. Microtomes are an… …   Wikipedia

  • Post-SSRI sexual dysfunction — (PSSD) [Bahrick AS. [http://www.division55.org/Tablet/Vol7No3.pdf Post SSRI Sexual Dysfunction.] American Society for the Advancement of Pharmacotherapy Tablet 2006; 7:2 10.] is an iatrogenic type of sexual dysfunction caused directly by the… …   Wikipedia

  • ATP6AP1 — ATPase, H+ transporting, lysosomal accessory protein 1, also known as ATP6AP1, is a human gene.cite web | title = Entrez Gene: ATP6AP1 ATPase, H+ transporting, lysosomal accessory protein 1| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene… …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”