NEK2

NIMA (never in mitosis gene a)-related kinase 2
PDB rendering based on 2jav.
Available structures PDB 2JAV, 2W5A, 2W5B, 2W5H, 2WQO, 2XK3, 2XK4, 2XK6, 2XK7, 2XK8, 2XKC, 2XKD, 2XKE, 2XKF
Identifiers
Symbols	NEK2; HsPK21; NEK2A; NLK1
External IDs	OMIM: 604043 MGI: 109359 HomoloGene: 74441 GeneCards: NEK2 Gene
EC number	2.7.11.1
Gene Ontology Molecular function • nucleotide binding • protein kinase activity • protein serine/threonine kinase activity • protein binding • ATP binding • protein phosphatase binding • metal ion binding Cellular component • kinetochore • kinetochore • condensed chromosome kinetochore • condensed nuclear chromosome • spindle pole • nucleus • chromosome • nucleolus • cytoplasm • centrosome • cytosol • cytosol • midbody • protein complex Biological process • mitotic sister chromatid segregation • G2/M transition of mitotic cell cycle • mitotic cell cycle • protein phosphorylation • chromosome segregation • mitosis • regulation of mitosis • meiosis • protein autophosphorylation • centrosome separation • cell division Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4751	18005
Ensembl	ENSG00000117650	ENSMUSG00000026622
UniProt	P51955	Q3TF15
RefSeq (mRNA)	NM_001204182.1	NM_010892.3
RefSeq (protein)	NP_001191111.1	NP_035022.2
Location (UCSC)	Chr 1: 211.84 – 211.85 Mb	Chr 1: 193.65 – 193.66 Mb
PubMed search	[1]	[2]

Serine/threonine-protein kinase Nek2 is an enzyme that in humans is encoded by the NEK2 gene.^[1][2]

Interactions

NEK2 has been shown to interact with MAPK1^[3] and NDC80.^[4][5]

References

1. ^ Schultz SJ, Nigg EA (Feb 1994). "Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans". Cell Growth Differ 4 (10): 821–30. PMID 8274451. 
2. ^ "Entrez Gene: NEK2 NIMA (never in mitosis gene a)-related kinase 2". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4751. 
3. ^ Lou, Yang; Xie Wei, Zhang Dong-Fang, Yao Jian-hui, Luo Zhao-feng, Wang Yu-Zhen, Shi Yun-Yu, Yao Xue-Biao (Aug. 2004). "Nek2A specifies the centrosomal localization of Erk2". Biochem. Biophys. Res. Commun. (United States) 321 (2): 495–501. doi:10.1016/j.bbrc.2004.06.171. ISSN 0006-291X. PMID 15358203. 
4. ^ Chen, Yumay; Riley Daniel J, Zheng Lei, Chen Phang-Lang, Lee Wen-Hwa (Dec. 2002). "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation". J. Biol. Chem. (United States) 277 (51): 49408–16. doi:10.1074/jbc.M207069200. ISSN 0021-9258. PMID 12386167. 
5. ^ Chen, Y; Sharp Z D, Lee W H (Sep. 1997). "HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins". J. Biol. Chem. (UNITED STATES) 272 (38): 24081–7. doi:10.1074/jbc.272.38.24081. ISSN 0021-9258. PMID 9295362. 

Further reading

• Hayward DG, Fry AM (2006). "Nek2 kinase in chromosome instability and cancer.". Cancer Lett. 237 (2): 155–66. doi:10.1016/j.canlet.2005.06.017. PMID 16084011. 
• Schultz SJ, Fry AM, Sütterlin C, et al. (1994). "Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans.". Cell Growth Differ. 5 (6): 625–35. PMID 7522034. 
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298. 
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. 
• Fry AM, Meraldi P, Nigg EA (1998). "A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators". EMBO J. 17 (2): 470–81. doi:10.1093/emboj/17.2.470. PMC 1170398. PMID 9430639. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170398. 
• Fry AM, Mayor T, Meraldi P, et al. (1998). "C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2". J. Cell Biol. 141 (7): 1563–74. doi:10.1083/jcb.141.7.1563. PMC 2133000. PMID 9647649. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2133000. 
• Fry AM, Arnaud L, Nigg EA (1999). "Activity of the human centrosomal kinase, Nek2, depends on an unusual leucine zipper dimerization motif". J. Biol. Chem. 274 (23): 16304–10. doi:10.1074/jbc.274.23.16304. PMID 10347187. 
• Schutte BC, Bjork BC, Coppage KB, et al. (2000). "A preliminary gene map for the Van der Woude syndrome critical region derived from 900 kb of genomic sequence at 1q32-q41". Genome Res. 10 (1): 81–94. doi:10.1101/gr.10.1.81. PMC 310500. PMID 10645953. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310500. 
• Helps NR, Luo X, Barker HM, Cohen PT (2001). "NIMA-related kinase 2 (Nek2), a cell-cycle-regulated protein kinase localized to centrosomes, is complexed to protein phosphatase 1". Biochem. J. 349 (Pt 2): 509–18. doi:10.1042/0264-6021:3490509. PMC 1221174. PMID 10880350. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1221174. 
• Hames RS, Fry AM (2002). "Alternative splice variants of the human centrosome kinase Nek2 exhibit distinct patterns of expression in mitosis". Biochem. J. 361 (Pt 1): 77–85. doi:10.1042/0264-6021:3610077. PMC 1222281. PMID 11742531. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1222281. 
• Ha Kim Y, Yeol Choi J, Jeong Y, et al. (2002). "Nek2 localizes to multiple sites in mitotic cells, suggesting its involvement in multiple cellular functions during the cell cycle". Biochem. Biophys. Res. Commun. 290 (2): 730–6. doi:10.1006/bbrc.2001.6212. PMID 11785960. 
• Eto M, Elliott E, Prickett TD, Brautigan DL (2003). "Inhibitor-2 regulates protein phosphatase-1 complexed with NimA-related kinase to induce centrosome separation". J. Biol. Chem. 277 (46): 44013–20. doi:10.1074/jbc.M208035200. PMID 12221103. 
• Chen Y, Riley DJ, Zheng L, et al. (2003). "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation". J. Biol. Chem. 277 (51): 49408–16. doi:10.1074/jbc.M207069200. PMID 12386167. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Faragher AJ, Fry AM (2004). "Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles". Mol. Biol. Cell 14 (7): 2876–89. doi:10.1091/mbc.E03-02-0108. PMC 165683. PMID 12857871. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=165683. 
• Lou Y, Yao J, Zereshki A, et al. (2004). "NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling". J. Biol. Chem. 279 (19): 20049–57. doi:10.1074/jbc.M314205200. PMID 14978040. 
• Noguchi K, Fukazawa H, Murakami Y, Uehara Y (2004). "Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells". J. Biol. Chem. 279 (31): 32716–27. doi:10.1074/jbc.M404104200. PMID 15161910. 
• Fortna A, Kim Y, MacLaren E, et al. (2006). "Lineage-specific gene duplication and loss in human and great ape evolution". PLoS Biol. 2 (7): E207. doi:10.1371/journal.pbio.0020207. PMC 449870. PMID 15252450. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=449870. 
• Lou Y, Xie W, Zhang DF, et al. (2004). "Nek2A specifies the centrosomal localization of Erk2". Biochem. Biophys. Res. Commun. 321 (2): 495–501. doi:10.1016/j.bbrc.2004.06.171. PMID 15358203.